Ponik Lab publishes new methods protocol to use endogenous fluorescence to segment intravital images.

May 2022. This novel method demonstrates an intravital imaging workflow pairing collagen second harmonic generation imaging, endogenous fluorescence from the metabolic co-factor NAD(P)H, and fluorescence lifetime imaging microscopy (FLIM) as a means to non-invasively compartmentalize the tumor microenvironment into basic domains of the tumor nest, the surrounding stroma or ECM, and the vasculature.

Read the full article at: https://www.jove.com/t/63413/a-label-free-segmentation-approach-for-intravital-imaging-mammary