May 2022. This novel method demonstrates an intravital imaging workflow pairing collagen second harmonic generation imaging, endogenous fluorescence from the metabolic co-factor NAD(P)H, and fluorescence lifetime imaging microscopy (FLIM) as a means to non-invasively compartmentalize the tumor microenvironment into basic domains of the tumor nest, the surrounding stroma or ECM, and the vasculature.
Read the full article at: https://www.jove.com/t/63413/a-label-free-segmentation-approach-for-intravital-imaging-mammary