Lee HJ, Gari MK, Inman DR, Rosenkrans ZT, Burkel BM, Engle JW, Hernandez R, Kwon GS*, Ponik SM*. Multimodal Imaging Demonstrates Enhanced Tumor Exposure of the Fibronectin-targeting PEGylated Functional Upstream Domain Peptide in Breast Cancer.

Burkel BM, Inman DR, Virumbrales-Muñoz M, Hoffmann EJ, and Ponik SM.   A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment  J. Vis. Exp. (183), e63413, doi:10.3791/63413 (2022).

Buck K, Roberts D, Aballo T, Inman D.R, Song J, Ponik SM, Brown K, and Ge Y.  “One-Pot Exosome Proteomics Enabled by a Photocleavable Surfactant. Anal Chem. 2022 May 24;94(20):7164-PMID:35543580

Iida T, Mizutani Y, Esaki N, Ponik SM, Burkel BM, Weng L, Kuwata K, Masamune A, Ishihara S, Haga H, Kataoka K, Mii S, Shiraki Y, Ishikawa T, Ohno E, Kawashima H, Hirooka Y, Fujishiro M, Takahashi M, Enomoto A. Pharmacologic conversion of cancer-associated fibroblasts from a protumor phenotype to an antitumor phenotype improves the sensitivity of pancreatic cancer to chemotherapeutics. Oncogene 2022 May;41(19):2764-2777. PMID: 35414659

Fertal SA, Poterala JE, Ponik SM, Wisinski KB. Stromal characteristics and impact on new therapies for metastatic triple negative breast cancer. Cancers (Basel). 2022 Feb 27;14(5):1238. PMID:35267548


Lugo-Cintrón KM, Ayuso JM, Humayun M, Gong MM, Kerr SC, Ponik SM, Harari PM, Virumbrales-Muñoz M*, and Beebe DJ*. Primary Head and Neck Tumour-Derived Fibroblasts Promote Lymphangiogenesis in a Lymphatic Organotypic Co-culture Model. e-Biomedicine 2021 Nov.

Adams MT, Dwulet JM, Briggs JK, Reissaus CA, Jin E, Szulczewski JM, Lyman MR, Sdao SM, Kravets V, Nimkulrat SD, Ponik SM, Merrins MJ, Mirmira RG, Linnemann AK, Benninger RK, Blum B. Reduced synchroneity of intra-islet Ca2+ oscillations in vivo in Robo-deficient β cells. Elife. 2021 Jul 7;10:e61308. doi:10.7554/eLife.61308. PMID: 34231467

Proestaki M, Burkel BM, Galles EE, Ponik SM, Notbohm J. Effect of matrix heterogeneity on cell mechanosensing.  Soft Matter. 2021 Jun 14. doi: 10.1039/d1sm00312g. PMID: 34125129

Szulczewski JM, Inman DR, Proestaki M, Notbohm J, Burkel BM, and Ponik SM. Directional Cues in the Tumor Microenvironment due to Cell Contraction against Aligned Collagen Fibers. Acta Biomaterialia, ePub May, 7 2021

Macdonald EB, Begovatz P, Barton GP, Erickson-Bhatt S, Inman DR, Cox BL, Eliceiri KW, Strigel RM, Ponik SM, Fain SB. Hyperpolarized 13C Magnetic Resonance Spectroscopic Imaging of Pyruvate Metabolism in Murine Breast Cancer Models of Different Metastatic Potential. Metabolites. 2021 Apr 27;11(5). PubMed PMID: 33925445.

Humayun M, Ayuso JM, Brenneke RA, Virumbrales-Muñoz M, Lugo-Cintrón K, Kerr S, Ponik SM, Beebe DJ. Elucidating cancer-vascular paracrine signaling using a human organotypic breast cancer cell extravasation model. Biomaterials. 2021 Mar;270:120640. PMID: 33592387


Curran CS, Ponik SM.  The Mammary Tumor Microenvironment. Adv Exp Med Biol. 2020;1296:163-181. doi: 10.1007/978-3-030-59038-3_10. PMID: 34185292

Knott SJ, Brown KA, Josyer H, Carr A, Inman D, Jin S, Friedl A, Ponik SM, Ge Y. Photocleavable Surfactant-Enabled Extracellular Matrix Proteomics. Anal Chem. 2020 Dec 15;92(24):15693-15698. PMID: 33232116

Hoffmann EJ, Ponik SM. Biomechanical Contributions to Macrophage Activation in the Tumor Microenvironment. Front Oncol2020;10:787doi: 10.3389/fonc.2020.00787. eCollection 2020. Review. PubMed Central PMCID: PMC7251173.

Lugo-Cintrón KM, Gong MM, Ayuso JM, Tomko LA, Beebe DJ, Virumbrales-Muñoz M, Ponik SM. Breast Fibroblasts and ECM Components Modulate Breast Cancer Cell Migration Through the Secretion of MMPs in a 3D Microfluidic Co-Culture Model. Cancers (Basel)2020 May 6;12(5)doi: 10.3390/cancers12051173. PubMed Central PMCID: PMC7281408.

Sung BH, von Lersner A, Guerrero J, Krystofiak ES, Inman D, Pelletier R, Zijlstra A, Ponik SM, Weaver AM. A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells. Nat Commun2020 Apr 29;11(1):2092doi: 10.1038/s41467-020-15747-2. PMCID: PMC7190671.

Lugo-Cintrón KM, Ayuso JM, White BR, Harari PM, Ponik SM, Beebe DJ, Gong MM, Virumbrales-Muñoz M. Matrix density drives 3D organotypic lymphatic vessel activation in a microfluidic model of the breast tumor microenvironment. Lab Chip2020 May 7;20(9):1586-1600doi: 10.1039/d0lc00099j. PubMed PMID: 32297896


Mizutani Y, Kobayashi H, Iida T, Asai N, Masamune A, Hara A, Esaki N, Ushida K, Mii S, Shiraki Y, Ando K, Weng L, Ishihara S, Ponik SM, Conklin MW, Haga H, Nagasaka A, Miyata T, Matsuyama M, Kobayashi T, Fujii T, Yamada S, Yamaguchi J, Wang T, Woods SL, Worthley D, Shimamura T, Fujishiro M, Hirooka Y, Takahashi M, Enomoto A. Meflin-positive cancer-associated fibroblasts inhibit pancreatic carcinogenesis. Cancer Res. 2019 Aug 22.  PMID: 31439548

Jallow F, O’Leary KA, Rugowski DE, Guerrero JF, Ponik SM, Schuler LA. Dynamic interactions between the extracellular matrix and estrogen activity in progression of ER+ breast cancer. Oncogene2019 Oct;38(43):6913-6925doi: 10.1038/s41388-019-0941-0. PubMed Central PMCID: PMC6814534.

Berger AJ, Renner CM, Hale I, Yang X, Ponik SM, Weisman PS, Masters KS, Kreeger PK. Scaffold stiffness influences breast cancer cell invasion via EGFR-linked Mena upregulation and matrix remodeling. Matrix Biol. 2019 Jul 16 PMID:31323325

Cox BL, Erickson-Bhatt S, Szulczewski JM, Squirrell JM, Ludwig KD, Macdonald EB, Swader R, Ponik SM, Eliceiri KW, Fain SB. A novel bioreactor for combined magnetic resonance spectroscopy and optical imaging of metabolism in 3D cell cultures. Magn Reson Med. 2019 May;81(5):3379-3391. PMID: 30652350


Tomko,LA, Hill RC, Barrett A, Szulczewski JM, Conklin MW, Eliceiri KW, Keely PJ, Hansen KC, Ponik SM. Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma. Sci Rep. 2018 Aug 28;8(1):12941  PMCID: PMC6113240

Esbona K, Yi Y, Saha S, Yu M, Van Doorn RR, Conklin MW, Graham DS, Wisinski KB, Ponik SM, Eliceiri KW, Wilke LG, Keely PJ. The presence of COX-2, tumor-associated macrophages, and collagen alignment as prognostic markers for invasive breast carcinoma patients. Am J Pathol. 2018 Mar;188(3):559-573   PMCID:PMC5963475


Ishihara S, Ponik SM, Haga H. Mesenchymal stem cells in breast cancer: response to chemical and mechanical stimuli.  Oncoscience. 2017 Nov 28;4(11-12):158-159. doi: 10.18632/oncoscience.381. eCollection 2017 Nov. No abstract available. PMID:29344547

Ishihara S, Inman DR, Li W, Ponik SM, Keely PJ. Mechano-signal transducation of mesenchymal stem cells induce mammary cancer growth via prosaposin secretion.  Cancer Res. 2017 Sep 28 PMCID: PMC5816983


Morris BA, Burkel B, Ponik SM*, Fan J, Condeelis JS, Aguire-Ghiso JA, Castracane J, Denu JM, Keely PJ. Collagen Matrix Density Drives the Metabolic Shift in Breast Cancer Cells. EBioMedicine. 2016 Nov;13:146-156.2016. PMCID: PMC5264313 (*Corresponding author)

Corsa CA, Brenot A, Grither WR, Van Hove S, Loza AJ, Zhang K, Ponik SM, Liu Y, DeNardo DG, Eliceiri KW, Keely PJ, Longmore GD.The Action of Discoidin Domain Receptor 2 in Basal Tumor Cells and Stromal Cancer-Associated Fibroblasts Is Critical for Breast Cancer Metastasis. Cell Rep. 2016 Jun 14;15(11):2510-23.  PMCID: PMC4909540

Zhang K, Grither WR, Van Hove S, Biswas H, Ponik SM, Eliceiri KW, Keely PJ, Longmore GD. Mechanical signals regulate and activate SNAIL1 protein to control the fibrogenic response of cancer-associated fibroblasts. J Cell Sci. 2016 May 15;129(10):1989-2002 PMCID: PMC3794710

García-Mendoza MG, Inman DR, Ponik SM, Jeffery JJ, Sheerar DS, Van Doorn RR, Keely PJ.  Neutrophils drive accelerated tumor progression in the collagen-dense mammary tumor microenvironment.  Breast Cancer Res. 2016 May 11;18(1):49. PMCID: PMC4864897

Burkel B, Morris BA, Ponik SM, Riching KM, Eliceiri KW, Keely PJ.  Preparation of 3D Collagen Gels and Microchannels for the Study of 3D interactions In Vivo.  J Vis Exp. 2016 May 9;(111). PMCID: PMC4942088  jove-protocol-preparation of 3d collagen gels in microchannels

Szulczewski JM, Inman DR, Entenberg D, Ponik SM, Aguirre-Ghiso J, Castracane J, Condeelis J, Eliceiri KW, Keely PJ.  In Vivo Visualization of Stromal Macrophages via label-free FLIM-based metabolite imaging. Sci Rep. 2016 May;6:25086. PMCID: PMC4879594


Curran CS, Carrillo ER, Ponik SM, Keely PJ. Collagen density regulates xenobiotic and hypoxic response of mammary epithelial cells. Environ Toxicol Pharmacol. 2015 Jan;39(1):114-24  PMID 25481308

2014 (and prior)

Riching KM, Cox BL, Salick MR, Pehlke C, Riching AS, Ponik SM, Bass BR, Crone WC, Jiang Y, Weaver AM, Eliceiri KW, Keely PJ. 3D collagen alignment limits protrusions to enhance breast cancer cell persistence. Biophys J. 2014 Dec 2;107(11):2546-58.

Ponik SM, Trier SM, Woznik MA, Eliceiri KW, Keely PJ.  RhoA is down-regulated at cell-cell contacts via p190RhoGAP-B in response to tensional homeostasis.  Mol Biol Cell. 2013 Jun;24(11):1688-99, S1-3. doi: 10.1091/mbc.E12-05-0386.

Zhang K, Corsa CA, Ponik SM, Prior JL, Piwnica-Worms D, Keely PJ, Eliceiri KW, Longmore GD. The Collagen Receptor Discoidin Domain Receptor 2 Stabilizes Snail1 Protein to Facilitate Breast Cancer Metastasis.  Nat Cell Biol. 2013 Jun;15(6):677-87. doi: 10.1038/ncb2743. 

Gehler S, Ponik SM, Riching KM, Keely PJ. Bi-directional signaling: extracellular matrix and integrin regulation of breast tumor progression. Crit Rev Eukaryot Gene Expr. 2013;23(2):139-57. Review.

Heck JN, Ponik SM, Garcia-Mendoza MG, Pehlke CA, Inman DR, Eliceiri KW, Keely PJ. Microtubules regulate GEF-H1 in response to extracellular matrix stiffness.  Mol Biol Cell. 2012 Jul;23(13):2583-92. 

Ajeti V, Nadiarnykh O, Ponik SM, Keely PJ, Eliceiri KW, Campagnola PJ.  Structural changes in mixed Col I/Col V collagen gels probed by SHG microscopy: implication for probing stromal alterations in human breast cancer.  Biomed Opt Express. 2011 Aug 1;2(8):2307-16.

P.P. Provenzano, C.T. Rueden, S.M. Trier, L.Yan, S. M. Ponik, D.R. Inman, P.J. Keely, and K.W. Eliceiri.  Nonlinear Optical Imaging and Spectral-Lifetime Computational Analysis of Endogenous and Exogenous Fluorophores in Breast Cancer.  J Biomed Opt. 2008 May-Jun;13(3):031220

P.J. Keely, M.W. Conklin, S. Gehler, S.M. Ponik, and P.P. Provanzano.  Investigating Integrin Regulation and Signaling Events in Three-Dimensional Systems.  Methods in Enzymology 425, Chapter 2, 2007